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Pivotal role of melatonin plus exercise as a neuroregenerator following focal cerebral ischemic injury
Author(s) -
Park Sookyoung,
Lee Seunghoon,
Hong Yonggeun
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.lb723
Subject(s) - melatonin , neun , medicine , neurogenesis , penumbra , doublecortin , endocrinology , lesion , stroke (engine) , neuroregeneration , ischemia , neuroprotection , dentate gyrus , anesthesia , neuroscience , hippocampus , pathology , psychology , immunohistochemistry , mechanical engineering , engineering
Among the numerous studies related to therapeutic mechanism following stroke, neurogenesis plays a critical role. Therefore, we hypothesized that combination therapy with melatonin and exercise might exert on the neurogenesis after focal cerebral ischemia. Sprague‐Dawley rats were randomly divided into control (Con) and middle cerebral artery occlusion (MCAo) groups treated vehicle (Veh), melatonin (MT), exercise (EX), and melatonin with treadmill exercise (MT+Ex). MCAo‐induced neurological deficits were improved by melatonin with and without exercise. Furthermore, we confirmed the expression of NeuN and Doublecortin were significantly reduced in MCAo+Veh, while melatonin with without exercise attenuated these alterations. The expression of BDNF, in particular mature‐BDNF was increased, but not pro‐BDNF after MCAo. These changes were reduced at chronic phase of MCAo. GFAP expression was increased in lesion area of MCAo+Veh, then melatonin with and without exercise decreased these changes. By histological examination, MCAo‐induced infarction volumes were reduced by melatonin with and without exercise. We found that neurite length from perikaryon was markedly diminished than control rat. However, melatonin plus exercise promotes neurite outgrowth from penumbra to infarct core lesion. Thus, these results demonstrate that melatonin plus exercise could contribute to neuroregeneration and support the potential use for stroke. Funding: 2014R1A1A3051724, 2013 Post‐Doctoral Research Program of Inje University, 2012R1A1A2005089, 2013R1A2A2A01067169, KGM4611512