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Influence of N ‐Acetylcysteine on Apoptosis in White‐Gastrocnemius Muscle of Rats With Chronic Heart Failure
Author(s) -
Martinez Paula,
Lopes V,
Pagan L,
Bonomo C,
Gomes M,
Oliveira Junior S,
Cezar M,
Damatto R,
Lima A,
Fernandes D,
Laurindo F,
Zornoff L,
Okoshi K,
Okoshi M
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.lb710
Subject(s) - medicine , gastrocnemius muscle , acetylcysteine , analysis of variance , myocardial infarction , apoptosis , dna fragmentation , skeletal muscle , heart failure , cardiology , antioxidant , endocrinology , chemistry , biochemistry , programmed cell death
The aim of this study is to evaluate the influence of the antioxidant N ‐acetylcysteine (NAC) on apoptosis in skeletal muscle of rats with myocardial infarction (MI)‐induced HF. Methods: MI was induced by left coronary occlusion. Four months later, rats were assigned to three groups: Sham (n=7), MI‐C (MI without treatment, n=9), and MI‐NAC (MI treated with N ‐acetylcysteine, 120 mg/kg/day, n=10). Rats with small MI were excluded. Six months after surgery, reactive oxygen species generation was assessed by HPLC analysis of dihydroethidium (DHE) oxidation fluorescent products, 2‐hydroxyethidium (EOH) and ethidium, in gastrocnemius muscle (white portion). Apoptosis was analyzed by quantification of DNA fragmentation using an ELISA kit. Results MI size did not differ between MI‐C and MI‐NAC groups ( p >0.05; Student's t test). EOH/DHE ratio was higher in MI‐C than in Sham and MI‐NAC (Sham 21.3±4.9; MI‐C 46.3±24.5; MI‐NAC 25.9±10.4 nM/µM/g tissue; p =0.014; ANOVA and Bonferroni). The ethidium/DHE ratio was similar between groups. DNA fragmentation was higher in MI‐C than Sham and similar in MI‐C and MI‐NAC (Sham 1.37±0.48; MI‐C 2.85±1.65; MI‐NAC 2.60±0.92 arbitrary units/g tissue; p =0.045; ANOVA and Bonferroni). Conclusion NAC administration does not attenuate apoptosis in gastrocnemius muscle of heart failure rats. Financial support: FAPESP and CNPq.