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The Influence of Excess Postexercise Leucine Ingestion on Markers of Autophagy in Skeletal Muscle of Older Men
Author(s) -
D'Lugos Andrew,
Reidy Paul,
Gundermann David,
Borack Michael,
Volpi Elena,
Rasmussen Blake,
Dickinson Jared
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.lb680
Subject(s) - skeletal muscle , leucine , endocrinology , autophagy , medicine , ingestion , vastus lateralis muscle , messenger rna , western blot , basal (medicine) , sarcopenia , chemistry , biology , amino acid , biochemistry , gene , apoptosis , insulin
The goal of this study was to observe the role of excess post exercise leucine ingestion on the autophagy system in skeletal muscle following resistance exercise (RE) in old adults over a 24h time course. Older men performed a bout of RE and at 1h postexercise ingested 10g of essential amino acids (EAA) containing a leucine content similar to high quality protein (CTRL, 1.8g leucine, n=7) or excess leucine (LEU, 3.5g leucine, n=8). Western blot (protein) and RT‐qPCR (mRNA expression) were used to examine markers of autophagy in skeletal muscle ( vastus lateralis ) biopsies obtained at rest and at 2, 5, and 24h postexercise. Relative to basal values, mRNA expression of Beclin1 was increased (1.3 fold) in both groups at 5h and 24h post exercise (P<0.05). BNIP3 expression was increased (~1.3 fold) only in LEU at 5h (P<0.05). GABARAP mRNA expression was unchanged in both groups and at all time points (P>0.05). LC3BI protein increased at 5h post exercise for both groups (P<0.05), whereas LC3BII was unchanged (P>0.05). The ratio of LC3BII/LC3BI was reduced in both groups at all time points postexercise (P<0.05), which is associated with a decrease in autophagic flux. These data indicate that autophagy may be reduced in skeletal muscle following the combination of RE and EAA ingestion and that markers associated with autophagy in the skeletal muscle of older men do not appear to be influenced by excess postexercise leucine ingestion. NIAMS R01AR049877, NIA P30AG024832, NIH T32HD07539, NCATS UL1TR000071