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Effect of Acute Bouts of High Intensity Exercise in Hot and Cold Environments on Autophagy, Oxidative, and Inflammatory Proteins in Neutrophils
Author(s) -
Lewis James,
Greska Eric,
CosioLima Ludmilla,
Lee Youngil
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.lb679
Subject(s) - autophagy , immune system , western blot , heat shock protein , innate immune system , cytokine , oxidative stress , inflammation , immunology , medicine , hsp70 , endocrinology , biology , biochemistry , apoptosis , gene
Moderate exercise has been shown to increase the body's immune cells and their activity. Circulating blood neutrophils (NEUTs) play an important role in being the first respondents of the innate immune system. Understanding neutrophil's response to varying body temperatures during exercise by examining changes in stress proteins, may reveal novel information as to how the human body adapts to stressful environments, continues normal cellular function and initiates the immune system response. The purpose of this study was to determine whether intense bouts of exercise in different environmental conditions induces autophagy and cytokine responses in neutrophils. Male human subjects (n=9), (22+2 years old) cycled at 70% VO 2 max for 30 minutes during two separate exercise sessions in an environmental chamber set at 15 °C on the first session and 32°C in the second session. Blood samples were taken before (PRE), immediately after (POST), and one hour after (1H POST) each session. NEUTs were isolated and examined using Western Blot analysis to determine changes in specific protein expression. Heat shock protein (HSP72), cytokine (TNF‐a), autophagy proteins (LC3‐II) and ubiquitinated proteins were quantified. No significant changes in proteins were observed between treatments. These findings may suggest that 30 minutes of intense cycling is not strenuous enough to produce significant changes in autophagy and inflammatory activation. Our results indicate that short bouts of intense exercise in stressful environments (hot vs. cold) may be safe and not cause unnecessary inflammatory responses.