Premium
Synthesis of type I collagen in an animal model of skin injury via TGF‐β1 and IGF‐1R upregulation
Author(s) -
Choi Jeonghyun,
Hong Yonggeun
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.lb635
Subject(s) - wound healing , granulation tissue , transforming growth factor , downregulation and upregulation , connective tissue , growth factor , fibroblast , myofibroblast , angiogenesis , cytokine , microbiology and biotechnology , transforming growth factor beta , extracellular matrix , inflammation , immunology , medicine , biology , pathology , cancer research , cell culture , fibrosis , receptor , biochemistry , gene , genetics
Many approaches that accelerate the wound healing process have been developed. However, improving the speed and quality of healing remain a challenging task faced by clinicians. Collagens are an important component in all phage of wound healing. They impart integrity and strength to all tissue. Transforming growth factor‐beta1 (TGF‐β1) is important in inflammation, angiogenesis, reepithelialization, and connective tissue regeneration. This play a role in wound contraction by facilitating fibroblast contraction of the collagen matrix. Insulin‐like growth factor‐1 (IGF‐1) is a cytokine that participates in the cellular granulation process during wound healing. IGF‐1 has also described as an agent that acts anabolically and anti‐catabolically in a wide variety of cell type. The aim of this study was to evaluate whether upregulation of TGF‐β1 and IGF‐1R related to tissue repair process of wound in an animal model. Skin injury were created 1.5㎠ on dorsal right and left. Animal were housed in ad libitum and controlled with a photoperiod of 12h. On postoperative 3h, 15h, 2d, 7d, and 10d the complete wound with a 0.5 ㎝ margin was carefully removed. TGF‐β1 gene expression was increased in 2d, 7d, and 10d. IGF‐1R gene expression was showed in the same manner. Synthesis of type I collagen was growing as time dependent. These finding suggest that typeIcollagen synthesize was influenced by TGF‐β1 and IGF‐1R. Funding: 2012R1A1A2005089, 2013R1A2A2A01067169, KGM4611512