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Obesity in Saturated Fat Diet Does Not Alter the Proteins Involved in Myocardial Calcium Transit
Author(s) -
Deus Adriana,
Vileigas Danielle,
Silva Danielle,
Campos Dijon,
Santana Paula,
Oliveira Scarlet,
Pagan Luana,
Okoshi Marina,
Cicogna Antonio
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.lb630
Subject(s) - phospholamban , medicine , endocrinology , calsequestrin , ryanodine receptor , calcium , triglyceride , chemistry , obesity , cholesterol
The aim of this study was to test the hypothesis that obesity by saturated high‐fat diet leads to changes in the expression and / or phosphorylation of proteins related to myocardial calcium handling. Wistar rats were divided into two groups: control (C, n = 18; saturated normolipidic diet) and obese (OB; n = 19; saturated high‐fat diet) for 30 weeks. Obesity was determined by adiposity index and comorbidities were evaluated. Cardiac morphological profile was estimated by post‐death macroscopic analysis. The protein expressions of calcium pump of the sarcoplasmic reticulum (SERCA2a), ryanodine receptor (RYR), calsequestrin (CSQ), phospholamban total (PLB), phospholamban serine 16 (PLB be 16), phospholamban threonine 17 (PLB thr 17), Na + /Ca 2+ exchanger (NCX) and L‐type calcium channel (L channel) were performed by the method of Western Blot. In this study, the animals showed significant increase in adiposity index (p<0.001) and several comorbidities such as glucose intolerance (p<0.001), hyperleptinemia (p<0.001), hyperinsulinemia (p=0.03), hypertriglyceridemia (p=0.001), increased LDL (p=0.03) and NEFA (p=0.02) and hypertension (p=0.009). Macroscopic results showed no significant cardiac remodeling (p>0.05). The analysis of proteins related to the calcium handling showedno significant change: PLB (p=0.71), CSQ (p=0.62), SERCA2a (p= 0.61), PLB 17 Thr (p=0.58), NCX (p=0.47), PLB is 16 (p=0.42), RYR (p=0.19) and channel L (p=0.10). In conclusion, the results of this study show that obesity by saturated high‐fat diet does not promote significant changes in the levels of proteins related to myocardial calcium handling. Suport Fapesp.