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Imbalance between matrix metalloproteinase (MMP)‐2 and calponin‐1 in early hypertensive vascular remodeling
Author(s) -
Belo Vanessa,
TanusSantos José Eduardo,
Castro Michele
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.lb595
Subject(s) - calponin , medicine , matrix metalloproteinase , blood pressure , endocrinology , renovascular hypertension , vascular remodelling in the embryo , cardiology , immunohistochemistry
Increased matrix metalloproteinase (MMP)‐2 contributes to hypertension‐induced chronic vascular remodeling. Calponin is a contractile protein, which is cleaved by MMP‐2, and a differentiation marker of vascular smooth muscle cells (VMSCs). Reduced calponin levels are associated with phenotype switch which leads to migration and remodeling. We examined whether there is a relationship between MMP‐2 and calponin‐1 with arterial remodeling in the early and chronic phases of two‐kidney, one‐clip (2K1C) hypertension. Systolic blood pressure (SBP) was assessed in sham and 2K1C rats at 1, 2 and 10 weeks by tail‐cuff plethysmography. Aortic MMP‐2 and calponin‐1 were determined by Western blot. Quantitative morphometry was performed in aortic sections to analyze structural changes. SBP increased from 152 ± 8 mmHg in 1 week of hypertension to 180 ± 8 mmHg and 194 ± 3 mmHg in 2 and 10 weeks (p<0.05 vs. Sham). We observed increased MMP‐2 and reduced calponin‐1 in aortas of 2K1C rats at 1 week of hypertension (P<0.05 r=‐0.74), followed by early, non‐significant changes in arterial structure. Increased MMP‐2 and calponin were observed at 2 and 10 weeks of hypertension (P<0.05), which were accompanied by increased aortic media thickness and media/lumen (P<0.05). These results indicate that an imbalance between MMP‐2 and calponin‐1 occurs only in early hypertension and may be a starting point of chronic arterial remodeling. Fundação de Amparo a Pesquisa do Estado de São Paulo, Conselho Nacional de Desenvolvimento Científico e Tecnológico.