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Sevoflurane Increases Proliferation, Adhesion on HUVEC and Incorporation in Tubular Structures of Endothelial Progenitor Cells
Author(s) -
Munteanu Vlad Adelina,
Isvoranu Gheorghita,
Gilca Marilena,
Ceafalan Laura,
Surcel Mihaela,
Stoian Irina,
Manda Gina
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.lb590
Subject(s) - sevoflurane , progenitor cell , apoptosis , andrology , peripheral blood mononuclear cell , endostatin , chemistry , anesthetic , endothelial progenitor cell , microbiology and biotechnology , immunology , in vitro , medicine , pharmacology , endothelial stem cell , stem cell , angiogenesis , biology , anesthesia , biochemistry
As a pursuit of our previous finding that anesthetic preconditioning (APC) increases plasma and tissue levels of endothelial progenitors (EPCs) in rodents, we investigate here the effects of sevoflurane on proliferation and function of EPCs. Cultures of human cord blood mononuclear cells were exposed to sevoflurane 2% or 4% in air/5% CO 2 , or only to air/5% CO 2 (sham control). The number of double staining cells for DiIAcLDL and UEAI‐FITC/20x microscopic field was increased following exposure to sevoflurane 2% (180.62 ± 19.09, n = 4, p<0,05) and 4% (167.36 ± 7.95, n = 4, p<0,05) versus control (123.98 ± 11.17). LDH cytotoxicity assay showed no significant effects of the anesthetic on EPCs, and a tetrazolium salt test indicated raised proliferation 24 hrs post‐exposure to sevoflurane 4% (0.156±0.024 vs. 0.124±0.011, n=4, p<0.05). Apoptosis was lowered, but without statistic significance. Adherence of DiI labelled EPCs to HUVEC monolayer was enhanced 24 hrs postexposure to sevoflurane 2% (58.28 ± 13.39 vs. 27.67 ± 5.15, n=4, p<0.05) and 4% (65.22 ± 13.16 vs. 23.35 ± 3.75, n=4, p<0.05), and incorporation in tubules of HUVEC was increased as well (sevoflurane 4%: 97.12±3.61 vs. 47.6±1.94, n=3, p<0.01). Thus, augmented EPCs proliferation, ability to adhere to endothelial cells and to incorporate in vascular structures are candidate mechanisms underlying the regenerative and anti‐ischemic processes of APC with sevoflurane. Financial support: research grant PN‐II‐RU‐TE‐2012‐3‐0463, UEFISCDI Romania.