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Investigation of the Properties of the CART Receptor in PC‐12 Cells
Author(s) -
Prinster Steven
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.lb506
Subject(s) - cart , receptor , biology , microbiology and biotechnology , cocaine and amphetamine regulated transcript , amphetamine , phosphorylation , peptide , paracrine signalling , endocrinology , medicine , dopamine , neuropeptide , biochemistry , mechanical engineering , engineering
The cocaine‐ and amphetamine‐regulated transcript (CART) was first isolated from rat striatum following the acute administration of cocaine or amphetamine. It was quickly determined that CART or CART peptide was expressed in a variety of tissues affecting a wide range of important processes. The function of these areas have grown to include feeding behavior, pleasure and reward, diabetes, ovarian follicle development, bone remodeling and anxiety. In spite of the relevance of these conditions as therapeutic targets, development of small molecules to modulate these systems has been impeded by the lack of an identified receptor for CART peptide. Localization studies suggest that CART and CART peptide are located in similar areas: hypothalamus, pituitary and adrenal gland. Cells isolated from these tissues have provided information delineating much of the current state of knowledge regarding the CART peptide receptor. We have characterized the signaling pathways activated by CART peptide in rat PC‐12 cells. We observed a pertussis‐toxin sensitive alteration of cAMP production, and phosphorylation of extracellular signal regulated kinase 1 and 2. Effects were also observed on the phosphorylation of protein kinase B. These results provide addition evidence that the receptor responsible for mediating the effect of the CART peptide is a G i/o ‐coupled receptor. These CART peptide studies provide important information that will aid in the identification of CART peptide receptor.