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Viability of the Bilayer Bacterial Cellulose Membrane as a Biological Support for use in Tissue Engineering and Regenerative Medicine
Author(s) -
Sartori Kamila,
Lararini Silmara,
Carvalho Renata,
Barud Hernane,
Lustri Wilton,
Amaral André
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.lb49
Subject(s) - bilayer , tissue engineering , microbiology and biotechnology , viability assay , regenerative medicine , biomaterial , chemistry , cell , membrane , biophysics , biology , stem cell , biomedical engineering , biochemistry , nanotechnology , materials science , engineering
Tissue engineering is an interdisciplinary scientific field that seeks to restore or improve the biological functions of damaged tissue or unable to perform its function. Some biomaterials are used as three‐dimensional structures, which act as biological media, enabling and stimulating cell proliferation and differentiation and consequent induction of tissue reconstruction. The bacterial cellulose membrane (BCm) bilayer represents a promising biomaterial for use as a biological support. However, there are no reports on their permissiveness for membership and maintenance of cellular homeostasis. This research aimed to evaluate the potential of BCm bilayer to the development and maintenance of cell viability. For this, BCm and mono bilayer were cultured for 4 days in 24‐well plates and processed cultured with osteoblast lineage (OSTEO‐I). The morphological aspects of adhesion and spreading of cells, assessed by microscopic analysis, have been established for 10 days after sowing. The results demonstrated the feasibility of BCm monolayer for membership, spreading and expanding the cell quota, causing the gradual coating the surface of the membranes. However, the BCm bilayer present in both layers, a substantially lower viability, suggesting a limited capacity for use as a biological support cellularized. Although it has promising structural features, the BCm bilayer appears to provide fewer favorable conditions for membership and maintenance of cellular homeostasis.