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Dynamics of stem cells in skin flaps subjected to ischemia reperfusion injury
Author(s) -
Tang Ya Hui,
Pennington Lindsey,
Scordino Jessica,
Alexander J,
Lian Timothy
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.lb47
Subject(s) - medicine , ischemia , perfusion , reperfusion injury , pathology , intravital microscopy , wound healing , mesenchymal stem cell , stromal cell , bone marrow , stem cell , soft tissue , surgery , microcirculation , biology , genetics
Reconstructive surgery of the head and neck in cancer or trauma patients frequently involves the use of soft tissue flaps. Ischemia reperfusion injury (IRI) is an important cause of failure in skin flaps. In a mouse model we have previously reported that treatment of skin flaps subjected to IRI with bone marrow stromal cells (BMSC) decreases tissue injury and enhances of wound healing resulting in improved flap survival. Objective Characterization of BMSC and leukocyte dynamics within the microvasculature of skin flaps subjected to IRI using fluorescence and intravital microscopy in an established mouse model. Methods Skin flaps based on the inferior epigastric artery were raised on C57BL/6 mice and were subjected to 3.5 hours of ischemia and then reperfused. Leukocytes labeled with rhodamine‐6G. BMSC labeled with (CFSE) were administered intravenously. The rolling and firm adhesion of both cell populations was monitored in arterioles and venules. Results Both leukocytes and BMSC were noted to accumulate in the skin flap vasculature after IRI; however, may more leukocytes were recruited than BMSC. The appearance of BMSC and leukocytes in the postischemic flap microvasculature was associated with a diminished vessel perfusion rate compared to controls. Conclusion BMSC are recruited to skin flaps subjected to IRI. The characteristics of BMSC and leukocyte activity in the microvasculature of skin flaps defined in this study may be utilized to further investigating the mechanism of action BMSC in skin flaps subjected to IRI.