Premium
Effects of Glucose Deprivation on The Biological Phenotypes of Human Malignant Mesothelial Cells
Author(s) -
Kim HangAh,
Kim MyungChul,
Kim NaYon,
Seo YuRi,
Kim Yongbaek
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.lb462
Subject(s) - glucose transporter , cancer cell , glycolysis , biology , phenotype , glucose uptake , anaerobic glycolysis , cytotoxic t cell , tumor microenvironment , cancer research , cancer , carbohydrate metabolism , cell culture , endocrinology , microbiology and biotechnology , metabolism , biochemistry , in vitro , gene , tumor cells , genetics , insulin
Microenvironment is important in the maintenance of biological phenotypes such as survival, proliferation, and migration capacity in cancer cells. Decreased concentration of glucose is a common feature of the tumor microenvironment and has been reported to play a critical role in the acquirement of malignancy.Cancer cell metabolism is shifted toward glycolysis by the increased expression of glycolytic enzymes, glucose transporters, and inhibitors of mitochondrial metabolism. This study was performed to evaluate the effects of glucose‐free on the phenotypes of human malignant mesothelioma (HMM) cells. Glucose free media decreased the cell survival and proliferation of HMM cells. The mobility and invasion capacities were significantly decreased by glucose deprivation. These characteristics of cancer cells were resulted from the promotion of apoptosis. Gene expression level of ABCG2, an efflux transporter of cytotoxic drugs, was increased in glucose free media compared to normal media. The results suggest that deprivation of glucose from cancer cells affect their biological phenotypes due to lack of energy and increase multidrug resistance.