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A Scanning Acoustic Microscope ‐ A Novel Tool for Cytological Discrimination
Author(s) -
Miura Katsutoshi,
Tsuchida Takashi,
Yamamoto Seiji
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.lb459
Subject(s) - cell , microscope , cell size , pathology , staining , scanning acoustic microscope , cell type , materials science , microscopy , biomedical engineering , chemistry , acoustic microscopy , biology , medicine , microbiology and biotechnology , biochemistry
A scanning acoustic microscope (SAM) uses ultrasound to make cell images on the slide by plotting the data of cell thickness, speed of sound (SOS), and attenuation of sound (AOS) through cells without staining. We have applied this microscope to discriminate various free cells in the fluid for cytological diagnosis. Purpose How different are SAM images of various free cells from optical ones? Is SAM image analysis applicable to cytological diagnosis? Materials and Methods Residual cells of cytology specimen from body cavity were prepared to make single cell‐layer slides by liquid‐based cytology method. The slides were fixed in ethanol and kept dry until observation. The slide were soaked in distilled water and observed with SAM. Cells from squamous cell carcinomas, adenocarcinomas, reactive mesothelial cells, mesotheliomas, malignant lymphomas, and neutrophils /macrophages were compared of their SOS, AOS, and thickness. Results SAM could visualize not only SOS, AOS and thickness of each cell portion but also cell sizes, thicknesses, borders, contents, cell clusters and structures. Various free cells showed their own characteristic acoustic images that was equivalent to optic ones. Obtained data with SAM was statistically comparable among different cell types.Cytological analysis SAM LM Nucleus Size △ ◎Irregularity/groove X ◎chromatin density X ◎ Cytoplasm Size ◎ ○Border ◎ ○Content ◎ ○Irregularity ◎ △ Cell connection/Cluster ◎ ◎ Structure ◎ ◎ Statistical analysis ◎ ○Discussion Each acoustic property of different cell types was distinct to make characteristic acoustic images. Statistical difference in SOS, AOS and thickness of each cell type may help discriminate cell types. SAM can be a novel diagnostic tool for cytological diagnosis.