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Profiling the Metabolome Changes Caused by Cranberry Proanthocyanidins in Female Rats using a UPLC‐Q Exactive‐MS Based Global Metabolomics Approach
Author(s) -
Liu Haiyan,
Garrett Timothy,
Gu Liwei
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.lb341
Subject(s) - proanthocyanidin , metabolome , chemistry , metabolomics , metabolite , glucuronide , cranberry juice , hydroxybenzoic acid , food science , biochemistry , chromatography , polyphenol , biology , endocrinology , antioxidant , urinary system , organic chemistry
The overall metabolic changes in female rats fed with cranberry proanthocyanidins were investigated using a global UPLC‐Q Exactive‐MS based metabolomics approach. Twenty four female Sprague‐Dawley rats were randomly separated into two groups and received proanthocyanidins extracted from cranberries or apples by oral gavage for 3 times using a 250 mg/kg body weight dose each. Serum samples were collected 4hours after the last gavage and analyzed using UPLC‐Q Exactive‐MS followed by multivariate analyses. Metabolome in serum of rats fed with cranberry proanthocyanidins differed from those fed with apple proanthocyanidins. Cranberry proanthocyanidins caused a greater increase in hydroxybenzoic acid, phenol, cathechol sulphate, dihydroxyphenylvaleric acid, 4'‐ O ‐methyl‐(‐)‐epicatechin‐3'‐ O ‐beta‐glucuronide in rat serum. Furthermore, the serum level of several exogenous metabolites including peonidin‐3‐ O ‐hexose, methylated epicatechin glucuronide and sulphated dihydroxyphenylvaleric acid were higher in rats fed with apple proanthocyanidins. The metabolomic changes caused by cranberry proanthocyanidins may help to explain its reported health benefits and identify biomarkers for dietary cranberry intake.