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German Chamomile ( Matricaria chamomilla ) Extract and Its Major Polyphenols Inhibit Intestinal α‐glycosidases i n vitro
Author(s) -
VillaRodriguez Jose,
Kerimi Asimina,
Abranko Laszlo,
Williamson Gary
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.lb323
Subject(s) - apigenin , polyphenol , chemistry , ferulic acid , matricaria chamomilla , acarbose , in vitro , biochemistry , pharmacology , traditional medicine , antioxidant , flavonoid , enzyme , medicine
Excessive post‐prandial glucose concentration is associated with an increased risk of developing type 2 diabetes. Polyphenols represent a biologically active class of molecules which have been tested as supporting or alternative therapies for a number of chronic diseases. In this work we assessed the acarbose‐like activity of German chamomile extract (GCE) and related polyphenols on intestinal α‐glycosidases as a therapeutic approach for decreasing postprandial hyperglycemia. Qualitative and quantitative analysis of GCE was performed by HPLC‐ESI‐MS and HPLC‐DAD respectively, where four major polyphenols were identified namely cis ‐ and trans ‐ferulic acid hexoside, apigenin‐7‐ O ‐glucoside (A7G) and apigenin. Identification of ferulic acid hexosides was performed by accurate mass Q/TOFMS and their isomeric structures were confirmed by 1 H and 13 C NMR for the first time. The in vitro inhibition studies revealed that GCE and polyphenols inhibit salivary human α‐amylase (HSA) and maltase in a dose‐dependent manner; however their inhibitory effect was greater on HSA. Apigenin was the most potent inhibitor of HSA with IC 50 of 28 µM (p蠄0.05). When polyphenols were tested in combination at concentrations quantified in the extract, an additive effect was observed where A7G had the highest contribution (p蠄0.05). For maltase, A7G exhibited the highest inhibition (33%), nonetheless neither the GCE nor the polyphenols achieved 50% inhibition. The anti‐diabetic effect of German chamomile has been reported in the literature but the underlying mechanism is yet elusive. Our results suggest that this bioactivity could partially be mediated by the inhibition of intestinal glycosidases by the major polyphenols present.