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Intestinal Anti‐Inflammatory Activity Of Ginger Extract: Unveiling The Mechanisms In Colonic Epithelial Cells
Author(s) -
Kim Ji Yeon,
Kim Dong Min
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.lb278
Subject(s) - inflammation , ginseng , antioxidant , pharmacology , anti inflammatory , tumor necrosis factor alpha , chemistry , nf κb , proinflammatory cytokine , immunology , biochemistry , medicine , pathology , alternative medicine
Recently, several studies described the health‐promoting effects of ginger, including anti‐inflammatory properties, however the molecular mechanisms underlying its beneficial role remain largely unknown. Ginger is rich in phenolic compounds and it has been suggested that the positive effect of ginger intake might be attributed not only to the antioxidant properties of these compounds but also to the modulation of signaling cascades in connection with physiological and pathophysiological conditions such as inflammatory processes. This study assessed the potential anti‐inflammatory action of a ginger extract enriched in polyphenols in a cellular model of intestinal inflammation using cytokines‐stimulated Caco‐2 epithelial cells. In order to develop an in vitro model of the acute phase of intestinal inflammation, Caco‐2 cells were exposed to the mixture of inflammatory mediators such as IL‐1β, TNF‐α, IFN‐γand LPS,. Responses to the stimulus for the mixture of inflammatory mediators were assessed by measuring the secretion level of IL‐6 and IL‐8, Cox2 and iNOS mRNA expressions. In addition the effect for NF‐κB activation was measured. Ginseng extract and the containing compounds such as 6‐shogaol and 6‐gingerol were improved the inflammatory responses through decreasing NF‐κB activation. Ginger extract also ameliorated iNOS and Cox‐2 mRNA expression levels, significantly. These results shows that ginger extract may be developed as functional ingredients targeting gastrointestinal health.