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Systemic Nitric Oxide (NO) Production is Increased in Children with Sickle Cell Disease (SCD) Receiving Fortified Supplementary Food
Author(s) -
Marealle Alphonce,
Makani Julie,
Prentice Andrew,
Kamuhabwa Appolinary,
Sasi Philip,
Minzi Omary,
Siervo Mario,
Cox Sharon,
Wassell Sara
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.lb276
Subject(s) - medicine , arginine , nitric oxide , citrulline , pathophysiology , gastroenterology , amino acid , biochemistry , chemistry
Background Low bioavailable NO is implicated in the pathophysiology of SCD. Within a clinical trial of a daily ready‐to‐use supplementary food (RUSF) fortified with arginine (ARG, 0.2g/kg/d), the substrate of endothelial NO synthase, and citrulline (CITR, 0.1g/kg/d) vs . non fortified RUSF we conducted a nested feasibility study of systemic NO production. Methods Children (N=29, 70% male, 9‐11 years) with SCD underwent a modified Oral Nitrate Test (ONT) with reduced fasting time to measure the decay in serial saliva samples of an oral dose of labelled nitrate (Na 15 NO 3 ) by GC/MS [figure 1] (Siervo et al . 2011). Both groups received intervention for 12 weeks before the ONT and a further 5 weeks before plasma amino acids (Biochrom 30) and NO mediated endothelial function were assessed by flow mediated dilatation (FMD max ). Data were analysed using Wilcoxan ranksum or signrank tests.Results The intervention groups were similar for age and weight. NO production (µmol/kg/hr) was not associated with age or sex. Results by intervention group and for both intervention groups combined are presented in [figure 2]. No associations between NO production and amino acid concentrations or endothelial function (FMD max ) were observed.
Conclusions This method is feasible in young children in a low resource setting. NO production in malnourished children with SCD can be increased by protein energy supplementation and may be increased further with additional arginine and citrulline.