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Dietary IGF‐activated Plasma Protein Alleviates Intestine Dystrophy by Induction of Tight Junction Protein in Early Weaned Rats
Author(s) -
Kwak Minjin,
Kim Jonggun,
Kim HyungKwan,
Ha DongJin,
Whang KwangYoun
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.lb261
Subject(s) - crypt , jejunum , weaning , medicine , endocrinology , growth factor , insulin like growth factor , tight junction , biology , chemistry , biochemistry , receptor
Weaning is a stressful condition for mammals and negatively affects growth performance by dystrophy of intestinal villi and crypts. Dietary plasma protein (PP) has shown the increased growth performance in early‐weaned animals and it seems to be more than nutrient content itself. Among growth factors, dietary insulin‐like growth factor (IGF) known to enhance growth performance, but bound form of IGF might reduce growth promoting effects of IGF. The effects of IGF‐activated plasma protein (aIGFPP, by de‐binding with proteins) on growth performance and gut development were studied in early‐weaned rats. Twenty‐one rats (weaned at 14‐day) were allotted into three treatments (NC, NIH‐31 diet without animal proteins; PC, NIH‐31 diet with 50 g/kg PP; and IGF, NIH‐31 diet with 3.4 g/kg aIGFPP). After 6 days, jejunum samples were collected and gene expression of inflammatory cytokines (IL‐6, TNF‐α, IL‐1β, IL‐4, IL‐12β, and IL‐10) and tight junction proteins (β‐catenin and ZO‐1) were determined by qRT‐PCR. Body weights were measured daily. And the villus heights and crypt depths were also measured in jejunum. Body weight was numerically higher in rats of PC and IGF groups than rats of NC. There was no differences in TNF‐α, IL‐1β, IL‐4, IL‐12β, and IL‐10 expression, but IL‐6 was higher in IGF group than other groups (P<0.05). Tight junction protein, ZO‐1 and β‐catenin, expression was higher in IGF group than other groups at P<0.10 and P<0.05, respectively. Jejunal villus heights of rats in IGF group were higher than rats in other groups (P<0.05) but no difference was found in crypt depth. This data indicates aIGFPP could increase gut growth and might affect growth performance in early‐weaned rats.