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Effects of miR‐99b and miR‐365 on Osteoclast Differentiation
Author(s) -
Francis Cherylann,
Delany Anne,
Dole Neha,
Griggs Nathan
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.lb227
Subject(s) - osteoclast , rankl , microrna , microbiology and biotechnology , transfection , bone resorption , regulator , cellular differentiation , tartrate resistant acid phosphatase , biology , chemistry , gene , in vitro , biochemistry , genetics , activator (genetics)
MicroRNAs (miRNAs) are short (~22 nucleotide) non‐coding RNAs that regulate gene expression by decreasing the stability and/or translation of specific target mRNAs (Marco, 2011). There is a growing body of evidence suggesting that miRNAs play a critical role in the differentiation and function of osteoclasts, which are the cells responsible for bone resorption. Previously, we identified two miRNAs, miR‐99b and miR‐365, that were increased during osteoclast differentiation in vitro. In the present study, we evaluated the role of miR‐99b and miR‐365 in osteoclast differentiation by transfecting osteoclast precursors with inhibitors for these miRNAs. The effect of the inhibitors on RANKL‐mediated osteoclast differentiation was evaluated by staining for Tartrate‐Resistant Acid Phosphatase (TRAP), and quantifying TRAP‐positive multinucleated cells (osteoclasts). There was an increase in the number of osteoclasts in cells transfected with miR‐365 inhibitor, but decreased osteoclasts in cells transfected with miR‐99b inhibitor. Our results indicate that miR‐365 is a negative regulator of osteoclastogenesis, whereas miR‐99b is a positive regulator. microRNAs are key regulators of osteoclast differentiation.