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The Functions of miRNAs in Self‐renewal and Differentiation of Human Mesenchymal Stem Cells
Author(s) -
Suh Nayoung,
Park SeulKi,
Hwang Supyong,
Jung Yideun
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.lb193
Subject(s) - mesenchymal stem cell , microrna , bone morphogenetic protein 2 , microbiology and biotechnology , adipose tissue , stem cell , biology , cellular differentiation , cancer research , genetics , in vitro , gene , endocrinology
Human mesenchymal stem cells (hMSCs) have emerged as therapeutic promises for a wide range of diseases, but the regulatory mechanisms that control MSC fate determination and therapeutic efficacy remain poorly understood. MicroRNAs (miRNAs) are small non‐coding RNAs that regulate diverse biological processes at the post‐transcriptional level. We aimed to identify miRNAs enriched in hMSCs which modulate differentiation commitments. miRNA microarray analysis revealed that miR‐140‐3p and miR‐140‐5p were significantly upregulated in undifferentiated hMSCs from various tissue sources such as adipose, bone marrow, and umbilical cord, compared to human fibroblasts. Target prediction analysis and luciferase reporter assay revealed that miR‐140‐5p directly repress bone morphogenic protein 2 (BMP2), a potent inducer for osteogenetic differentiation. Furthermore, blocking miR‐140‐5p in human adipose‐derived mesenchymal stem cells (hADSCs) enhanced the expression of BMP2 and a subset of bone morphogenic protein (BMP) signaling components which is essential for osteogenetic lineage commitment. We propose that miR‐140‐5p can affect osteogenic lineage commitment in undifferentiated hADSCs at least in part by negatively regulate BMP2. §This work is supported by grants from KMEST (2012R1A1A3013555 and 2014R1A1A2059398).