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The Recruitment of the Transcription Factor YY1 to DNA Damage Sites in Human Cells
Author(s) -
Tesfazghi Merih,
Riman Sarah,
Alexander karen,
Rizkallah Raed,
Hurt Myra
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.lb186
Subject(s) - dna damage , dna repair , genome instability , yy1 , biology , microbiology and biotechnology , transcription factor , dna , genetics , human genome , gene , genome , promoter , gene expression
Our DNA is under continuous stress by endogenous and exogenous attacks such as genotoxic chemicals, ionizing radiation, and metabolic products. DNA damage is extremely detrimental, even a single unrepaired damage is sufficient to kill a cell. Cells mobilize a tightly regulated complex repair signaling pathway to either mend or remove the damaged DNA and avoid harmful consequences such as malignant transformation and genome instability syndromes. Here, for the first time, we show the rapid and transient recruitment of the transcription factor YY1 to laser induced damage sites in live human cells in real time, suggesting the early and direct role of YY1 in DNA repair. Use of a panel of YY1 deletion constructs reveal that part of the C‐terminus of the protein is required for the damage response and identified the repair response domain. Our findings provide first evidence for the early and potentially direct role of YY1 in DNA repair. To advance insight into the contribution of YY1 in DNA damage response, we continue to investigate the role of YY1 and its regulation in real time using different knockout human cells, inhibitors, and RNAi technology. Our findings will ultimately provide evidence on the mechanistic role of YY1 in DNA repair and further our understanding in the role of YY1 in genome integrity and maintenance.