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The Role of IL33 in Glioblastoma Multiforme
Author(s) -
Demircan Kadir,
Yukselten Yunus,
Bal Merve,
Bunsuz Merve,
Akcora Yildiz Dilara,
Sunguroglu Asuman,
Ugur Hasan
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.lb142
Subject(s) - microglia , glioma , cytokine , cancer research , malignancy , carcinogenesis , western blot , proinflammatory cytokine , u87 , cell culture , interleukin 6 , medicine , immunology , biology , cancer , pathology , inflammation , gene , biochemistry , genetics
Gliomas show the majority of primary brain tumors in adults and are part of the most malignant tumors. Although there are surgical resection and conventional therapy,Glioblastoma multiforme (GBM) patients' survivals are approximately 12‐15 months.Cytokines secreted by either cancer cells themselves or by the surrounding cells and infiltrating microglia/macrophages play a critical role in glioma malignancy. Lately IL‐33 is associated to tumorgenesis and are cytokines which play a key role on tumor pathogenesis. The aim of study is to search protein levels of IL‐33 and ADAMTS5, which is also effective at tumorigenesis in GBM.Western blot analysis showed over‐expression both of IL‐33 and ADAMTS5 in GBM.According to these results we showed that expression of IL‐33 and ADAMTS5 in GBM increased simultaneously. We treated cytokine of IL‐33 in GBM primary cell culture to prove these finding in vitro conditions. 0‐10‐30 ng/ml IL33 cytokine cells were treated for 48 h in order to show the effect on GBM primary cells. Exposure to IL‐33 treatment for 48 h resulted in dose dependent increase of ADAMTS5 protein levels in human GBM primary cells.Cytokines secreted by either cancer cells themselves or by the surrounding cells and infiltrating microglia/macrophages play a critical role in glioma malignancy. Our present findings, the abundant expression of proliferation‐associated IL‐33 is a key characteristic of human GBM primer cells, and these are involved in enhanced cell proliferation and tumorigenicity. Thus, the present study indicated that high expression of IL‐33 in glioblastoma cells may contribute to glioma progression.