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Association between DAPK1 Promoter Methylation and Cervical Cancer: a Systematic Review and a Meta‐analysis
Author(s) -
Agodi Antonella,
Barchitta Martina,
Quattrocchi Annalisa,
Maugeri Andrea,
Vinciguerra Manlio
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.lb133
Subject(s) - methylation , dna methylation , carcinogenesis , epigenetics , cervical cancer , promoter , meta analysis , biology , cancer , cancer research , oncology , medicine , gene , genetics , gene expression
DNA methylation, a major epigenetic mechanism, is involved in various biological processes including cancer. Particularly, promoter methylation can inactivate Death‐Associated Protein Kinase1 (DAPK1), a tumor suppressor gene which is often methylated during the carcinogenesis of cervical cancer. In the present systematic review and meta‐analysis, we evaluated the association between DAPK1 promoter methylation and cervical cancer risk. A literature search, using the PubMED database, was performed to select studies published up to July 2014. Subgroups analyses were performed. A total of 27 studies were included. All studies evaluated DAPK1 promoter methylation in squamous cell carcinoma and 12 studies also in adenocarcinoma. The “gold standard” method of promoter methylation evaluation was methylation specific PCR. Twenty studies, evaluating DAPK1 promoter methylation both in tumor and in healthy controls samples, were included in the meta‐analysis (1092 from cancer patients and 837 from controls). Meta‐analysis showed a significant association between DAPK1 promoter methylation and cervical cancer (pooled OR: 21.20; 95% CI: 11.14, 40.35) with the random effects model. The association was confirmed in specific subgroups. In conclusion DAPK1 promoter methylation might play an important role in the pathogenesis of cervical cancer. The promoter methylation of DAPK1 is a frequent event in cervical carcinogenesis and may have potential clinical application as a cancer biomarker. This work was conducted with the research support of Bench Srl, University of Catania.