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Viscum album var Hot Water Extract Mediates Anti‐cancer Effect through G1 Phase arrest in SK‐Hep1 Human Hepatocarcinoma Cells
Author(s) -
dela Cruz Joseph,
Kim Yeon Soo,
Hong Heeok,
Hwang SeongGu
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.lb129
Subject(s) - viscum album , cell cycle checkpoint , cell cycle , western blot , cyclin d1 , flow cytometry , cancer cell , viability assay , in vitro , cancer , traditional medicine , cell growth , biology , chemistry , cell , pharmacology , microbiology and biotechnology , medicine , biochemistry , gene
Viscum album var (VAV) also known as mistletoe, has long been categorized as a traditional herbal medicine in Asia. In addition to its immunomodulating activities, mistletoe has also been used in the treatment of chronic hepatic disorders in China and Korea. There are numerous reports showing that VAV possesses anti‐cancer effects, however, the effect against human hepatocarcinoma have never been elucidated.The hot water extracts of VAV was evaluated for its potential anti‐cancer effect in vitro. SK‐Hep1 cells (5 X 10 4 /well) were treated with VAV (50‐400ug/ml) for 24 hours, and then cell viability was measured. VAV dose dependently inhibited the proliferation of human hepatocarcinoma cells (SK‐Hep1). Flow cytometry analysis (FACS) of the cell cycle of SK‐Hep1 cells after a 48 hour treatment with various concentrations of VAV extract showed that VAV extract inhibited cell cycle of SK‐Hep1 cells via G1 phase arrest. RT‐PCR and Western blot analysis both revealed that CDK2 and Cyclin D1 gene expression were significantly down regulated while P21 was upregulated dose dependently by VAV treatment. These results indicate that VAV showed evidence of anti‐cancer activity through cell cycle arrest in SK‐Hep1cells.