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Anti‐Tumour Flavonoids Regulation on Oestrogen and Progesterone Receptor Genes in Cervical Cancer Cell Lines (HELA)
Author(s) -
Fadaka Adewale,
James Ayorinde,
Magbagbeola Olubunmi,
Alakija Abiola
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.lb128
Subject(s) - hela , progesterone receptor , cervical cancer , receptor , cancer , gene , cancer research , biology , cancer cell , oestrogen receptor , cell , chemistry , microbiology and biotechnology , biochemistry , breast cancer , genetics , estrogen receptor
Cervical cancer is the second most common cancer among women in the world. It is still the most common gynaecological cancer worldwide. Plants have remained an important source in the search for novel anti‐cancer compounds of which several polyphenolic flavonoids are interesting candidates. The present study was carried out to evaluate the modulatory effect of isolated flavonoids (free and bound) from Hibiscus Sabdariffa on oestrogen and progesterone receptor genes in cervical cancer cell lines (Hela). Growth inhibition concentration (IC 50 ) assay was done using MTT cell viability assay. Hela cells were treated with 200µg/ml of both free and bound flavonoids respectively. Total RNA was extracted and mRNA was converted to cDNA; semi‐quantitative polymerase chain reaction using densitometric analysis was carried out for both oestrogen and progesterone receptor genes. The results obtained showed that oestrogen receptor gene was down regulated by both the free and bound flavonoids while there was an up regulation of the progesterone receptor gene. In conclusion, this study therefore provides the scientific evidence for the therapeutic genetic modulation of sex hormone receptor genes of cervical cancer.