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Multiplex Analysis of Biomarkers in Thyroid Cancer
Author(s) -
Anderson Shaya,
Fuerstenberg Richard,
Brumbaugh Kathy,
Kalyuzhny Alex,
Sommer Stacy
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.lb127
Subject(s) - thyroid cancer , multiplex , thyroid , medicine , thyroid carcinoma , pathology , cancer , medullary carcinoma , biomarker , immunohistochemistry , chemokine , cancer research , oncology , biology , bioinformatics , inflammation , biochemistry
Thyroid cancer is the most common endocrine cancer, the incidence of which has been rapidly increasing over the last few decades. Traditionally, thyroid tumors are classified into two major groups based on morphologic and clinical features: undifferentiated (anaplastic) carcinomaand differentiated carcinoma (papillary, follicular and medullary). Early identification and diagnosis is essential for effective treatment. Cytokines, chemokines and growth factors play a critical role in the growth and differentiation of many target cells. These bio‐active molecules regulate the development and growth of both normal and neoplastic thyroid tissues. In addition, many cytokines and chemokines have been shown to generate antitumor responses. In patients with thyroid cancer, these molecules are often dysregulated, making them potentially useful as diagnostic biomarkers. In this study, thyroid carcinoma tissues were collected from patients diagnosed with papillary, follicular or medullary carcinoma. Paraffin‐embedded tissue sections were analyzed by IHC and adjacent frozen tissues were homogenized and assayed for biomarkers as large multiplexes using R&D Systems™ Luminex ® Screening Assays (Catalog# LxSAH). Initial testing was done using a multiplex of 27 relevant biomarkers. Results were verified by western blotting. The multiplex data was used to establish a biomarker profile for each of the three cancer types. In a follow up study, sera from five thyroid cancer patients and five apparently normal healthy individuals were evaluated using the same multiplexes. The serum results were compared to the tissue profiles with the intent of aiding in identification of the cancer type.