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Human Papillomavirus and sexually transmitted infections in women with and without abnormalities in cervical cytology
Author(s) -
Abreu Andre,
Souza Raquel,
Bonini Marcelo,
Gimenes Fabricia,
Consolaro Marcia
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.lb126
Subject(s) - chlamydia trachomatis , cytology , squamous intraepithelial lesion , medicine , genotype , gynecology , herpes simplex virus , gastroenterology , cervical intraepithelial neoplasia , virology , biology , cervical cancer , pathology , virus , cancer , gene , biochemistry
This report evaluated the molecular prevalence of HPV and sexually transmitted infections (STI) agentsas C. trachomatis, Herpes simplex virus (HSV)1/2 , N. gonorrhoeae, M. hominis, T. vaginalis and T. pallidum to contribute to the elucidation of STI infections in cervical carcinogenesis. Were recruited 828 Brazilian women between 15 to83 years old, 614 with normal and 224 with abnormal cytology (05 AGC/ atypical glandular cells; 65ASC‐US/atypical squamous cells of undetermined significance; 27ASC‐H – can't exclude high‐grade squamous intraepithelial cervical lesion; 77 LSIL/low‐grade SIL and 50 HSIL/high‐grade). The HPV was detected by polymerase chain reaction (PCR) and further genotyped by PCR‐RFLP (based restriction fragment length polymorphism analysis). STIs were detected by multiplex‐PCR. The HPV prevalence was 33.8%, n=284, showing correlation with abnormal (81.7% p = 0.0001) compared to normal cytology, 16.4%. The most frequent genotype was HPV‐16 (28.4%) in normal and abnormal cytology, followed by HPV‐31 ‐58 and ‐72 (7.9%, 6.9%, 6.0% respectively). HPV co‐infections were found in 26.7% and associated with abnormal cytology (p = 0.01). STI were detected in 30.4% (n= 255), 18.4% (n= 47) showed multiple agents: T. vaginalis 11.6% (n= 97), C. trachomatis 10.3% (n= 86), N. gonorrhoeae 4.7% (n= 39), HSV‐2 3.9% (n= 33), M. hominis 3.5% (n= 29), T. pallidum 1.7% (n=14), and HSV‐1 1.1% (n= 9). C. trachomatis was correlated with HPV especially with high‐risk regardless of cytology. This significant association suggests they may have synergistic pathological effects on cervical carcinogenesis, so it's important screening both microorganisms simultaneously than individually