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Metagenomic Profile of Fecal Samples Generated From Mouse Model Simulating Features of Post‐Traumatic Stress Disorder
Author(s) -
Gautam Aarti,
Hoke Allison,
Sitta Ramsey,
Sowe Bintu,
Dimitrov George,
Chakraborty Nabarun,
Muhie Seid,
Kumar Raina,
Hammamieh Rasha,
Jett Marti
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.lb104
Subject(s) - metagenomics , amplicon , feces , microbiome , 16s ribosomal rna , biology , traumatic stress , ribosomal rna , gut flora , computational biology , genetics , physiology , microbiology and biotechnology , medicine , polymerase chain reaction , gene , immunology , clinical psychology
Post‐traumatic stress disorder (PTSD) is an anxiety disorder that can arise due to exposure to a potentially traumatic event (PTE). Using “cage‐within‐cage resident‐intruder” protocol, subject C57BL/6J mice were exposed to aggressor mice for 10 days with 24 hours rest prior to harvesting. The symbiotic influence of microbiota on the nervous system and behavior has been receiving profound interest. In this longitudinal study, we are focusing on the effect of social stress on the microbiome by sequencing for 16S ribosomal RNA (16S rRNA) amplicon on the Illumina MiSeq platform. Fecal samples were collected at 2 day intervals during the 10 day protocol from control as well as subject mice. DNA was isolated using a kit from MO BIO followed by amplification of variable V3 and V4 region of the 16S rRNA amplicon using the Illumina 16S Metagenomics Library Preparation Protocol. The samples were barcoded using Nextera index primers and pooled in preparation for MiSeq sequencing. We will be able to characterize the fecal microbiota in the presence and absence of social stress and also will be able to correlate changes in bacterial communities in our mouse model of PTSD. DISCLAIMERS: Research was conducted in compliance with the Animal Welfare Act and all other Federal Requirements. The views expressed are those of the authors and do not constitute endorsement by the U.S. Army.