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MiR‐150 Expression Significantly Reduced in the Gut After Alcohol and Burn Injury
Author(s) -
Morris Niya,
Li Xiaoling,
Hammer Adam,
Can Abigail,
Earley Zachary,
Choudhry Mashkoor
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.998.9
Subject(s) - burn injury , total body surface area , intestinal permeability , alcohol , inflammation , small intestine , microrna , infiltration (hvac) , medicine , endocrinology , andrology , biology , immunology , pathology , physiology , surgery , gene , biochemistry , physics , thermodynamics
Alcohol is a major contributor of post burn pathology. Many of the adverse effects associated with alcohol and burn injury are linked to gut barrier disruption. The gut is the major reservoir of bacteria within the body; disruption of the gut barrier could result in bacterial translocation into extra‐intestinal sites. Recent studies demonstrated that alcohol and burn injury increases KC, IL‐6 and IL‐18 levels; which in turn could contribute to increased intestinal permeability. The mechanism underlying increased gut inflammation following alcohol and burn injury remains unknown. MicroRNAs (miR) are small noncoding RNAs that regulate gene expression. MiR‐150 is predicted to target IL‐18. Altered expression of miR‐150 could contribute to elevated IL‐18 and impaired gut permeability. Therefore, we examined miR‐150 and IL‐18 expression in small intestine epithelial cells (IEC) following alcohol and burn injury. Male mice (~25g) were gavaged with alcohol (~3 g/kg) or water. Four hours after the gavage, mice underwent a ~12.5% total body surface full thickness burn. One day following the injury, mice were euthanized; the small intestine was harvested and processed for IEC isolation. MiR‐150 expression was assessed by qRT‐PCR. IL‐18 levels were assessed by qRT‐PCR and ELISA. MiR‐150 expression was decreased by ~3‐fold following alcohol and burn injury. IL‐18 mRNA and protein levels were significantly increased following injury. The present study suggests that alcohol and burn injury reduces miR‐150 expression which contributes to elevated IL‐18. Studies are in progress examining if overexpression of miR‐150 reduces IL‐18 levels and improves gut barrier integrity. (R01AA015731, R01AA015731‐08S1, T32AA013527‐12)