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Centrifuge Size & Operation Effects On Mouse Adaptation To +G
Author(s) -
Fuller Charles,
HobanHiggins Tana,
Alberts Jeffrey
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.990.7
Subject(s) - centrifuge , rotation (mathematics) , centrifugation , radius , chemistry , rotational speed , adaptation (eye) , zoology , physics , chromatography , mathematics , biology , geometry , computer science , optics , computer security , quantum mechanics , nuclear physics
A centrifuge in space provides the only method to create environments between 0‐1G to understand the interactions between biological systems and gravity. Current designs for an on‐board centrifuge are short radius. However, the rotation rate to achieve +G increases as radius decreases, also enhancing irregularities in +G fields produced by centrifuge geometry and operation. Both centrifuge radius and rotation rate play a role in the adaptive response to altered G. This study examined the adaptation profiles of adult female mice to 8 weeks of chronic centrifugation. A rotation rate that would result in a 1.0 G environment in space was used to produce the maximum +G environment for this study. The +G field was either presented immediately upon centrifugation onset (acute) or in 4 equal increases (stepped) at two‐week intervals. Data were collected from: experimental (short 0.67 m diameter), force control (large 4.5 m diameter), cage control (the experimental cage) and vivarium control groups. Each group had an n=12 (4 cages of 3 subjects/group). Data included: biweekly measures of food and water consumption, body mass, body temperature, and video. The centrifuge groups showed the expected adaptation profiles to +G. Both the initial responses and the time to reach the adapted state were affected by the profile of centrifuge operation. The initial decrease in body mass, food consumption and body temperature was greater under the acute compared to the stepped profile. Additionally, the time to adapt was longer in the acute groups. However adaptation levels were similar after 8 weeks of +G. (NASA Grant TO1130JSSC.)