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Dipeptidyl‐peptidase IV mRNA Expression with Exercise and Amino Acid Supplementation
Author(s) -
Neidert Leslie,
Mobley Christopher,
Roberts Michael,
Kluess Heidi
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.989.3
Subject(s) - ctl* , endocrinology , medicine , dipeptidyl peptidase , messenger rna , skeletal muscle , chemistry , stimulation , dipeptidyl peptidase 4 , enzyme , biology , biochemistry , type 2 diabetes , in vitro , diabetes mellitus , cytotoxic t cell , gene
Dipeptidyl‐peptidase IV (DPP‐IV) is an enzyme circulating in the plasma, but the sources of plasma DPP‐IV are unknown. Plasma DPP‐IV is higher in individuals with high lean mass. Research showed that DPP‐IV is released from skeletal muscle as a myokine, making muscle a possible source of plasma DPP‐IV, but the stimulus for release is unknown. We hypothesized that acute exercise and amino acid or protein feeding would increase skeletal muscle DPP‐IV mRNA, and that the combination would have a synergistic effect. Male Wistar rats (~250 g) were fed 0.054 g leucine (LEU), 0.50 g whey (WP), or water (PLA) following 4 sets of 8 electrically‐stimulated unilateral plantarflexor contractions (EX). Contralateral un‐stimulated legs were used as a control condition (CTL). Gastrocnemius muscles were harvested 3 and 6 h post‐stimulation, real time PCR was performed to determine the change in DPP‐IV mRNA expression, and all data was normalized to PLA‐CTL muscles as fold‐change values. Relative to PLA‐CTL: a) PLA‐EX increased DPP‐IV mRNA 1.3‐fold at 3 h (p = 0.03); b) LEU‐CTL increased DPP‐IV mRNA 2.4‐fold at 3 h (p = 0.01), but declined to 1.3‐fold at 6 h (p = 0.27); c) LEU‐EX increased DPP‐IV mRNA 1.3‐fold at 3 h (p = 0.18), but fell below PLA‐CTL by 6 h (p = 0.12); d) WP‐CTL increased DPP‐IV mRNA 3.6‐fold by 3 h (p = 0.01) and remained 3.1‐fold elevated at 6 h (p = 0.001) ; and e) WP+EX increased DPP‐IV mRNA 1.9‐fold at 3 h (p =0.01) and remained elevated 3.1‐fold at 6 h (p = 0.01). DPP‐IV mRNA expression increased following muscle contraction and LEU and WP feeding, although WP appears to potently stimulate DPP‐IV mRNA expression regardless of muscle contraction. Future research should address if chronic WP supplementation chronically elevates DPP‐IV secretion from skeletal muscle.