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Neonatal Exercise and Age‐Related Oxidative Stress of the Rat Cerebral Cortex
Author(s) -
LopesdeMorais Andréia,
MendesdaSilva Rosâgela,
Ferreira Dioginis,
Pedroza Anderson,
AmânciodosSantos Angela,
Lagranha Claudia,
BatistadeOliveiraHornsby Manuella
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.983.9
Subject(s) - oxidative stress , medicine , endocrinology , catalase , malondialdehyde , treadmill , oxidative phosphorylation , antioxidant , brain aging , chemistry , biochemistry , disease
Aging is related to increased oxidative damage, since Harman proposed the “free‐radical theory of aging”. The cerebral tissue is strongly susceptible to oxidative stress (OS). Although, exercise can counteract OS by increasing anti‐oxidative defenses, it is not necessarily clear which factor of the exercise protocol is more critical. Furthermore, neonatal moderate exercise can decrease brain excitability in a long‐lasting manner (Batista‐de‐Oliveira et al 2012), however, the underlying mechanisms are still not clear. Therefore, we evaluated if the effects of neonatal moderate exercise is related to OS in cerebral cortexes of aged rats (585 days of age). At 15 days old, Wistar rats (n=20) were equally distributed in sedentary or exercised. The treadmill exercise was performed according to Batista‐de‐Oliveira et al (Exp Gerontology 47: 452 – 457, 2012). Malondialdehyde was measured as described by Buege & Aust (M Enzymol 52, 302‐310; 1978), Sulfhydryl content by G. Leipnitz et al. (Life Sciences 87 275–280; 2010), GSH levels by Hissin & Hilf (Analytical Biochemistry 74:214‐216; 1976), SOD activity by Misra & Fridovich (JBC 247(10): 3170‐3175), Catalase measured according to Aebi (M Enzymol 105, 121‐126; 1984), and GST by Nascimento et al. (APNM 39:1‐8). Neonatal exercise does not affect antioxidant enzymes in a long‐lasting manner (p > 0.05). Thereby, this exercise changes brain excitability later in life by other mechanism than increasing anti‐oxidative defenses. Study conducted in conformance with the FASEB Statement and the Institutional Ethics Committee for Animal Research (23076.028310/2012‐52)