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Early neonatal but not late neonatal sevoflurane inhalation induced special memory impairment revealed by behavioral investigation of mouse
Author(s) -
Hata Kanato,
Maekawa Masao,
Takasusuki Toshifumi,
Yamaguchi Shigeki,
Hori Yuuichi
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.983.2
Subject(s) - sevoflurane , morris water navigation task , inhalation , anesthesia , medicine , barnes maze , water maze , anesthetic , memory impairment , cognition , physiology , spatial learning , hippocampus , psychiatry
Sevoflurane is a volatile anesthetic that was developed relatively recently, and is now widely used in clinical anesthesia for infants. Sevoflurane has proposed effects on GABAa receptor‐mediated extrasynaptic tonic inhibition. Recent reports have shown that exposure to sevoflurane at an early age lead to long‐term cognitive dysfunction using animal models. However, the mechanisms underlying the sevoflurane induced cognitive dysfunction have not been fully understood. Aiming to find the mechanisms we observed behavioral changes of adult mice after the exposure to sevofrulane at different neonatal stage to define the period during which the sevoflurane exposure induces the long‐term cognitive dysfunction. Neonatal mice (3‐5 days, 12days, 20 days after birth) were exposed to 2% sevoflurane in O 2 for 4hrs. These mice were trained for 4 days (6 trials/day) from 49 days after birth for water maze test using a 1.2m diameter pool with a 10cm diameter platform. The escape latencies and the percent time of swim in the platform quadrant in the probe test for 60 sec was measured as indices of spatial memory formation. Mice exposed to sevoflurane at P3‐5 showed impaired water maze performance as compared with control mice. On the other hand, mice exposed to sevoflurane at P12 or P20 showed water maze performance comparable to the control mice. Our results suggest that during early neonatal period (around 3‐5 days after birth) the brain mechanisms relating spatial memory are sensitive to the sevoflurane exposure, but during later neonatal period (12 or 20 days after birth) those are not sensitive to the exposure.