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Cross‐Sensitivities of Amperometric Sensors Designed for Specific Gaseous Signaling Molecules
Author(s) -
DeLeon Eric,
Arif Maaz,
Huang Evelyn,
Gao Yan,
Olson Kenneth
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.979.11
Subject(s) - amperometry , chemistry , molecule , reagent , biophysics , organic chemistry , electrochemistry , biology , electrode
Biologically relevant gaseous signaling molecules, O 2 , NO, H 2 O 2 and H 2 S are often quantified with amperometric sensors (AS). Although AS are developed with reference to specificity regarding similar chemical moieties, rarely are they examined for cross‐sensitivity to dissimilar species. Here, we examine the specificity of three commercial AS (O 2 , NO, H 2 O 2 ) and an H 2 S AS built in‐house for these signaling molecules using known amounts of premixed O 2 , NO [via S‐Nitroso‐N‐ acetylpenicillamine (SNAP)], and reagent grade H 2 O 2 and Na 2 S using concentrations commonly cited in literature. We found that the H 2 S and O 2 AS were sensitive only to their respective gases, whereas the H 2 O 2 AS responded positively to both H 2 S and H 2 O 2 , and the NO AS responded to all of the chemicals tested. The H 2 O 2 AS was 25 fold more sensitive to H 2 S than H 2 O 2 , the NO AS was 15 fold more sensitive to H 2 S than NO and 3 fold more sensitive to H 2 O 2 than NO. Furthermore, the order of addition of these particular compounds altered the responses of each AS. The H 2 O 2 AS can be used on complex samples, but only in conjunction with an H 2 S AS, as determination of NO and H 2 O 2 requires significant back‐calculation; and even then results are only approximate. Conclusions O 2 and H 2 S AS are quite specific whereas there is substantial cross‐sensitivity when using NO and H 2 O 2 sensors. This can become especially problematic in tissues where multiple gaseous signaling molecules are generally present. Support: NSF DGE‐131‐3583 (ERD), IOS‐ 105‐1627 (KRO), IOS‐ 144‐6310 (KRO).