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Vanadium complexes inhibit the growth of HT‐29 and MCF‐7 cancer cell lines
Author(s) -
Sochor Victoria,
McCaffrey Vanessa,
Rabquer Bradley
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.978.7
Subject(s) - cell growth , mcf 7 , growth inhibition , apoptosis , cell culture , cancer cell , chemistry , cell cycle , in vitro , cell , cancer , cancer research , pharmacology , biology , biochemistry , medicine , human breast , genetics
Cancer is characterized by an over‐proliferation of cells that are no longer within control of the cell cycle. Platinum chemotherapeutic agents have been used effectively in cancer treatment but with adverse toxic effects. Recent studies suggest that complexes using vanadium (VCs) have effectively slowed or inhibited cancer cell growth through many methods, including the activation of apoptotic pathways. We synthesized novel VCs possessing different functional groups for this study. We hypothesized that these VCs act to inhibit cancer cell growth via induction of caspase 3/7. HT‐29 and MCF‐7 cell lines were used as models of colon and breast cancer, respectively. Three different VCs were synthesized, each having a unique functional group (5‐Br, 5‐OMe, 3‐OMe) that affected the electron density distribution within the VC. Using cell proliferation assays, efficacy of each VC was assessed in vitro . Treatments were applied for 24 hours in triplicate and the experiments were repeated on three separate occasions (n=3). VO 2 ‐Br was the most effective against both HT‐29 and MCF‐7 cell lines with 65.3% and 53.6% growth inhibition respectively. VO 2 ‐3‐OMe yielded 46.1% and 51.5% growth inhibition of HT‐29 and MCF‐7 cells, respectively. VO 2 ‐5‐OMe was the least effective, and yielded 13.4% growth in HT‐29 cells and 19.6% growth inhibition in MCF‐7 cells. Apoptosis assays were then performed on HT‐29 cell lines using 5‐Br to investigate possible growth inhibition mechanism. We observed no notable increase in caspase 3/7 activity of the treated groups compared to control groups. In conclusion, we found VCs to be effective at inhibiting growth of HT‐29 and MCF‐7 cell lines.