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Early Life Stress (ELS) Induces Dysregulation of Pro‐ and Anti‐Inflammatory Gene Expression in Renal Cortex of Adult Rats in Response to Chronic Angiotensin II
Author(s) -
Obi Ijeoma,
Loria Analia,
Ho Dao,
De Miguel Carmen,
Pollock Jennifer
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.965.5
Subject(s) - medicine , endocrinology , angiotensin ii , angiotensin ii receptor type 1 , receptor , inflammation , renal cortex , gene expression , chemistry , kidney , gene , biochemistry
ELS increases cardiovascular disease risk and chronic inflammation in human adults. We previously showed that maternal separation (MatSep), an ELS rat model, induces exaggerated blood pressure and renal cortical T‐cell infiltration in response to chronic AngII. We hypothesized that MatSep induces dysregulation of pro‐ and anti‐inflammatory gene expression in response to AngII infusion in adult rats. Expression of pro‐ and anti‐inflammatory genes was measured by PCR array or qRT‐PCR in circulating T cells, renal vessels, and renal cortices from control and MatSep rats. We found that at baseline MatSep significantly (p<0.05) induces CCL2, IL‐2R‐g, CD40 ligand, and TNFR1 genes in circulating T cells; CX3CL1, NFkB1 and TGFb in renal vessels; and caspase3, Flt3, IL‐15, Pik3r1, and Prlr in renal cortex when compared to control rats. In response to AngII infusion, renal cortices from control rats showed significant up‐regulation of mRNA expression of Cd1d1 (baseline vs. AngII, fold‐change, p<0.05: 1.0 + 0.04 vs. 1.9 + 0.30), Flt3 (1.0 + 0.30 vs. 1.9 + 0.20), and IL‐11 (1.0 + 0.30 vs. 3.5 + 0.60). In addition, control rats down‐regulated expression of interleukin 12‐b (1.0 + 0.30 vs. 0.2 + 0.10) and AT1 receptor (1.0 + 0.09 vs. 0.3 + 0.08), while MatSep rats showed an increase in AT1 receptor (1.1 + 0.20 vs. 2.4 + 0.40) in response to chronic AngII infusion. These data suggest that ELS induces sensitivity to chronic AngII infusion in adult rats via differential transcriptional responses. Funded by K99/R00 HL111354 to ASL, F32HL116145 to DHH, T32DK007545 to CDM, and P01HL69999 to JSP.