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Chronic fish oil supplementation partially reverted renal alterations observed in mice fed with a high fat diet
Author(s) -
Fernandez Ricardo,
Pequito Danielle,
Coelho Isabela
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.961.5
Subject(s) - endocrinology , medicine , renal function , excretion , creatinine , albuminuria , albumin , chemistry , fish oil , biology , fish <actinopterygii> , fishery
The objective of this study was evaluate the effect of chronic fish oil (FO) supplementation on renal morphology and function of mice fed with a high fat (HF) diet. Six weeks male mice were divided in two groups and fed during eight weeks with regular rodent chow (S), or HF diet. After this, they were supplemented orally for 30 days with FO, 1g/kg body weight. Renal function parameters, urinary excretion of TXB 2 , PGE 2 and albumin, immunolocalization of vimentin, desmin were measured. The weight gain of HF group was higher, almost 2 times, than S group. HF group present glucose intolerance, an effect that was not modified by FO. Plasma insulin levels increased significantly in HF. Creatinine clearance was lower in HF (0.81±0.09 mL/min/kg of body wt, n=31) if compared to S (1.28±0.22, n=20) (p<0.05), independent of FO supplementation. High‐fat diet induced a significant albuminuria (expressed as urine albumin/creatinine ratio): 35.65±7.32ng/mg, n=7 in HF vs 11.06±2.16 in S (p=0.01). FO supplementation reduced in 40% albumin excretion in HF (21.33±1.33). Urinary TXB 2 excretion was reduced in 58% by high‐fat diet and FO supplementation was not able to reverse this modification. Urinary excretion of PGE 2 was similar among groups. The high‐fat diet was able to increase in almost 100% the concentration of TNF‐a in renal tissue; an effect completely reverted by FO supplementation in tubular cells.The intrarenal expression of vimentin was significantly higher in the interstitium and tubules of HF group, an effect reverted by FO supplementation. Desmin expression in glomerulus edge increased in obese animals, but was not modified by FO supplementation. In summary, FO supplementation partially reverted renal alterations induced by HF diet.