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Bicarbonate Therapy Alleviates Hypertension‐Induced Renal Injury In Dahl Salt‐Sensitive Rats Independent of Systemic Blood Pressure
Author(s) -
Irsik Debra,
Ocasio Hiram,
Sun Jingping,
Chen JianKang,
O'Connor Paul
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.960.24
Subject(s) - blood pressure , renal function , medicine , excretion , bicarbonate , endocrinology , renal injury , medullary cavity , kidney , urinary system , sodium bicarbonate , mean arterial pressure , chemistry , heart rate
Lower serum HCO 3 ‐ concentrations are associated with a higher risk of progressive renal function loss and alkaline therapy improves renal outcomes in hypertensive patients. Experimental models are needed to better understand the mechanisms that mediate the beneficial outcomes of HCO 3 ‐ supplementation on the kidney. We tested whether bicarbonate supplementation attenuates hypertension‐induced renal injury in the Dahl salt‐sensitive (SS) rat model. 10 week old male Dahl SS rats received either 0.1 M NaHCO 3 or equimolar NaCl (control) given through drinking water over the course of the experiment (n=6‐7 rats/group). After 4 days of measurement on 0.4% NaCl (low salt (LS)) chow, rats were fed a high salt (8% NaCl (HS)) diet for 2 additional weeks. Blood pressure was continuously measured via radio‐telemetry and urine collected weekly. Urinary Na excretion was not different between groups. High salt feeding resulted in a similar increase in mean arterial pressure (MAP) in control (Day 14 HS; 142.5±6.8) and HCO 3 ‐ treated groups (Day 14 HS: 138.9±3.4; p=NS). Despite similar MAP responses, HCO 3 ‐ supplemented animals had reduced glomerular sclerosis (4.1±0.5 vs 8.3±2.0 % sclerosed glomeruli; P=0.052), outer medullary protein casts (1.2±0.3 vs 5.1±1.3 % medullary cast area; P=0.014) and 24 hour protein excretion on Day 14 of HS, 12.8±3.1 vs 47.4 ±18.7 mg day 14 HS; P=0.03) respectively, compared to control group. We conclude that HCO 3 ‐ supplementation protects from renal injury in high salt fed Dahl salt‐sensitive rats, independent of systemic blood pressure.