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Expression/Phosphorylation of the Renal NaCl Cotransporter (NCC) in Transplant Patients with Hypertension
Author(s) -
RojasVega Lorena,
Jimenez Aldo,
GomezOcadiz Ruy,
Alberú Josefina,
MoralesBuenrostro Luis,
Gamba Gerardo
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.960.22
Subject(s) - medicine , blood pressure , tacrolimus , urology , urinary system , ambulatory blood pressure , creatinine , endocrinology , lipid profile , renal function , kidney , body mass index , transplantation , gastroenterology , cholesterol
Tacrolimus‐induced hypertension in mice is associated with increased activity of the renal NaCl cotransporter (NCC). It is not known if this occurs in humans. Here, we performed a prospective, longitudinal and observational study to assess the prevalence of hypertension in patients undergoing renal transplantation and the expression/phosphorylation of NCC in urinary exosomes. From January 2013 all adult patients receiving a kidney allograft were enrolled. All patients received tacrolimus. At six and 12 months after surgery we assessed general clinical and laboratory variables, tacrolimus blood levels, and arterial blood pressure, which at six months was determined by ambulatory blood pressure monitoring (ABPM). At six and twelve months urinary exosomes were extracted to perform Western blot analysis using total and phospho‐NCC antibodies ( T45‐50‐55‐ NCC). At the moment 43 patients (69% male) have completed six months of follow up and ABPM. Mean age 36.7 ± 13.5 years. Of 17 women three had hypertension (17%), while of 26 males, 15 had hypertension (57%). Hypertensive men were older than normotensive (44.2 ± 14.2 vs. 30.7 ± 9.7, p<0.001), but body mass index, allograft rejection rate, serum tacrolimus and creatinine levels were similar. Western blot analysis has been performed in urinary exosomes from 9 hypertensive and 5 normotensive men. Values in normotensive men were taken as 1 and in hypertenvise normalized accordingly. Values in hypertensive vs. normotensive were: for NCC expression: 2.94 ± 0.5 vs. 1.0 ± 0.2 and pNCC 1.8 ± 0.4 vs. 1.0 ± 0.3, respectively (p<0.05). Our data suggest that NCC expression/phosphorylation is increased in hypertensive postranplant patients treated with tacrolimus.