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Intravital Imaging of the Kidney in Salt‐Sensitive Hypertension
Author(s) -
Palygin Oleg,
Endres Bradley,
Sandoval Ruben,
Rhodes George,
CamposBilderback Silvia,
Kamocka Malgorzata,
Molitoris Bruce,
Staruschenko Alexander,
Geurts Aron
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.960.12
Subject(s) - albuminuria , glomerulus , reabsorption , renal function , medicine , endocrinology , kidney glomerulus , kidney , albumin , renal physiology , renal glomerulus , chemistry , glomerulonephritis
Previous studies performed in rat kidneys shed light on the complexities of glomerular filtration of albumin and reabsorption at the proximal tubule (PT). It is now established that both PT reabsorption and transcytosis and glomerular filtration are responsible for determining the extent of albuminuria. Since hypertension is one of the most prevalent diseases worldwide, and is a major risk factor for developing albuminuria and renal failure, we investigated the mechanisms leading to albuminuria in the kidney of a rat model of salt‐sensitive hypertension, the Dahl SS rat. To determine the relative contributions of the glomerulus and PT to albuminuria in the SS rat, we applied the novel intravital two‐photon imaging to investigate the changes in renal function that occur during the development of salt‐sensitive hypertension (at 3, 7 and 14 days of high salt (HS) diet). Our results indicate that high amounts of albumin are filtered at the glomerulus in SS rats fed a low salt (LS) diet (GSC alb = 0.0155). Following HS diet consumption for 14 days, we observed significant elevations in blood pressure and albuminuria, increased prevalence of white blood cells (WBCs) within the kidneys (+Δ1.33 WBCs/image), and significant tubular damage as indicated by increased abundance of cast material (+Δ2.20 casts/image), compared to LS‐fed rats. We also observed a 57% increase in glomerular sieving of albumin (GSC alb = 0.0244) and a 58% reduction in PT reabsorption of albumin. Collectively, these results indicate that both the glomerulus and PT contribute to albuminuria in SS hypertensive rats.