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Cholinergic Stimulation with Nicotine Induces CD68+ Macrophage Infiltration into Kidney and Increases Arterial Pressure in Spontaneously Hypertensive Rats
Author(s) -
Harwani Sailesh,
Chapleau Mark,
Sutterwala Fayyaz,
Ballas Zuhair,
Patil Raju,
Meyerholz David,
Abboud Francois
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.957.7
Subject(s) - cd68 , endocrinology , medicine , stimulation , cholinergic , nicotine , spontaneously hypertensive rat , kidney , blood pressure , population , immunohistochemistry , environmental health
We have shown that cholinergic stimulation induces a pro‐inflammatory innate immune response in pre‐hypertensive Spontaneously Hypertensive rats (SHR). Here we hypothesized that cholinergic stimulation induces renal infiltration of a CD68+ macrophage population, contributing to renal hypertension. To test this, we infused either saline or nicotine (15 mg/kg/day) via osmotic pumps in pre‐hypertensive SHR and WKY over 2 weeks. We measured blood pressure (BP) by tail‐cuff and harvested renal tissue at 24 hours after initiating infusion. The presence of CD68+ macrophages in renal parenchyma was assessed by immunohistochemistry. Nicotine increased the number of renal CD68+ macrophages in SHR (n=3) from 47±8 to 73±7 cells per 400x field (p<0.001), but not WKY (34±8 cells vs 41±7 cells per 400x field) (p>0.05), without increasing the BP at 24 hours.However, over 2 weeks, nicotine increased the BP in pre‐hypertensive SHR from 123±4mmHg to 157±3mmHg (n=6, p<0.001), whereas saline did not (124±5mmHg vs 126±2mmHg, n=6, p>0.05). In contrast, nicotine had no effect on BP in the WKY.We conclude that cholinergic stimulation which is selectively pro‐inflammatory in innate immune cells of the pre‐hypertensive SHR also enhances CD68+ macrophage infiltration in the kidneys and may, thus, play a dual role in the development of hypertension in this genetic model of hypertension.