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Effect of Renal Denervation on Blood Pressure and MicroRNA 181a in Hypertensive Schlager Mice
Author(s) -
Head Geoffrey,
Gueguen Cindy,
Marques Francine,
Jackson Kristy,
Eikelis Nina,
Stevenson Emily,
Lambert Gavin,
Charchar Fadi,
Davern Pamela
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.957.1
Subject(s) - denervation , blood pressure , endocrinology , medicine , renin–angiotensin system , kidney , enalapril , sympathetic nervous system , norepinephrine , renal sympathetic denervation , chemistry , resistant hypertension , angiotensin converting enzyme , dopamine
Hypertensive Schlager mice (BPH) are hypertensive due to an exaggerated contribution of the sympathetic nervous system (SNS) and renin angiotensin system (RAS). The latter was associated with reduced expression of the renin regulatory micro RNA 181a. We therefore determined the effect of bilateral renal denervation (Rx) on blood pressure (BP) in BPH compared to normotensive BPN. BP was measured by radiotelemetry and Rx performed by surgery and 10% phenol. After 3 weeks, BP was 8±2 mmHg lower than sham in Rx BPH mice but Rx had no effect in BPN. Following Rx, enalapril (RAS contribution) decreased BP more in BPH mice compared to sham group but had no effect in BPN. The depressor response to pentolinium (SNS contribution) was greater in BPN mice following Rx compared with the sham group but the response was unaffected by Rx in BPH. Rx reduced renal norepinephrine levels in both strains but more so in BPH. Rx normalised both miR‐181a and renin mRNA in BPH to levels comparable to the control strain. We suggest that renal sympathetic activity is essential in maintaining hypertension in BPH mice partly by overexpression of renal renin as a result of inhibiting miR‐181a. Thus the antihypertensive effects of Rx may be dependent on the form of hypertension, whether it involves neuronal or renal mechanisms.