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The Role of Piezo1 in Regulating Cytosolic Calcium Level in Aortic Smooth Muscle Cells in Rats
Author(s) -
Zhang Jinglin,
Tjong Jeff,
Tang Nelson Leungsang,
Yao Xiaoqiang
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.943.3
Subject(s) - piezo1 , western blot , microbiology and biotechnology , cytosol , calcium , anatomy , mechanotransduction , medicine , chemistry , endocrinology , ion channel , biology , biochemistry , mechanosensitive channels , gene , receptor , enzyme
Piezo1 proteins are large integral proteins with 24‐39 transmembrane domains. Piezos are expressed in several tissues. Piezo ion channel family is evolutionarily conserved in mechanotransduction. We examined the expression level of Piezo1 in rat aortic smooth muscle cells (ASMCs) and determined stretch induced cytosolic calcium rise in these cells. Method mRNA and protein expressions of piezo 1 and 2 proteins in rat tissues were detected by RT‐PCR and western blot study, respectively. Stretch‐induced cytosolic calcium elevation in RASMC was determined by confocal microscopy. Results In rats, Piezo1 mRNA and protein was highly expressed in aorta, aortic smooth muscle cells and mesenteric artery, but low in cerebral artery, nodose ganglion and petrosal ganglion. Stretch‐induced [Ca 2+ ] i elevation was found to be increased in Piezo1 over‐expressing rat ASMCs compared with native rat ASMCs. Conclusion The results indicated that Piezo1 may play a functional role in stretch induced [Ca 2+ ] i elevation in rat arterial smooth muscle cell.