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Cytotoxicity and cell cycle effect on prostate cancer cell lines of Justicia spicigera hydroalcoholic extract
Author(s) -
Ramos Rodrigo,
Hernández María Elena,
Bravo Ivette,
Ramos Rafael,
Fernández Cynthia,
Sánchez Alberto,
Domínguez Miguel,
PérezPasten Ricardo,
Galar Marcela,
Godínez Marycarmen
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.936.9
Subject(s) - lncap , cell cycle , apoptosis , chemistry , cell cycle checkpoint , ic50 , cell , cell growth , mtt assay , cytotoxicity , cell culture , cytotoxic t cell , cancer cell , andrology , cancer , medicine , in vitro , biochemistry , biology , genetics
The aim of this research was to study the citotoxicity and cell cycle effect of hydroalcoholic extract of Justicia spicigera . The extract was obtained by maceration of aerials parts. Citotoxicity was evaluted by the MTT assay and cell cycle analysis was based on IC50 values at 12, 24 and 48 hours. The IC50 for DU‐145 and LNCaP was 285.9 μg/ml and 176.9 μg/ml respectively. Cell cycle analysis of DU‐145 showed a major percentage of cells in G0/G1 phase and decreased percentage of them in phase S at 24 h after treatment48 h post treatment increased Sub‐G0 phase and decreased G0/G1 phase proportionIn LNCaP cell line at 24 h, the G0/G1 phase increased and the G2/M phase decreasedAt 48 hours, the histograms exhibits a dramatic increase in the Sub‐G0 phase and decreased of the G2/M phaseThis results show the cytotoxic capacity of the extract and that LnCap cells were more sensitive compared to DU‐145 cells. Also ours results proved that the extract causes different effects depending on the cell line, while in LnCap cell the mayor event was cell death, in DU ‐145 cells G1/G0 arrest was visible, which may be due to synthesis arrest, but finally the effect depending on time of treatment in DU‐145 cells. Acknowledgments: CONACYT fellowship No. 290747, Cuerpo Académico de Neuroquímica UV‐CA‐304 and Cuerpo Académico Química Bioorgánica UV‐CA‐201.