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Cytotoxic activity and cell cycle effects on human breast cancer cell line (MCF7) of the hydroalcoholic extract of Justicia spicigera
Author(s) -
Bravo Ivette,
Hernández Elena,
Ramos Rodrigo,
Fernández Cynthia,
Ramos Rafael,
Sánchez Alberto,
PerezPasten Ricardo,
Galar Marcela,
Godinez Marycarmen,
Domínguez Miguel
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.936.2
Subject(s) - hacat , cell cycle , cell culture , cell cycle checkpoint , cytotoxic t cell , cytotoxicity , apoptosis , maceration (sewage) , cell , chemistry , cancer cell , ic50 , cancer , medicine , in vitro , biochemistry , biology , materials science , composite material , genetics
The aim of this work was to evaluate the effects of the hydroalcoholic extract of Justicia spicigera in a cancer cell line (MCF7) and compare it with a non‐carcinogenic cell line (HaCaT). Aerials parts of Justicia spicigera were extracted by maceration. The cytotoxicity assay was performed by the MTT assay. Cell cycle analysis was based on the IC 50 values at 12, 24 and 48 h. MCF7 cells obtained an IC 50 of 291.2 µg/ml and HaCaT cells an IC 50 349.2 µg/ml. Cell cycle distribution of HaCaT showed cycle arrest in G0/G1 phase at 24 h after treatment and at 48 h the cells arrested in the G0/G1 phase increase slightly on sub‐G0 percentageIn MCF7 cell cycle analysis showed arrest in G2/M phase at 24 h after treatment. However at 48 h, the cells arrested in the G2/M phase underwent cell death as evidences the increase on sub‐G0 percentageIt can be concluded that the extract exerts greater cytotoxic effect on cancer cell line MCF7 compared to HaCaT cell line. Moreover it induces an arrest at 24 hours in the G2/M phase of cell cycle, but at 48 h increased sub‐G0 phase (death) and compared with the results obtained from the cell line HaCaT, the Sub‐G0 phase did not increase drastically, which indicates that the extract may act selectively on cancer cell line. Acknowledgements: CONACYT No fellowship 289304, Cuerpo Académico de Neuroquímica UV‐CA‐304, Cuerpo Académico Química Biorgánica UV‐CA‐201.