Premium
Ferulic Acid Methylester from Tamarix aucheriana , Shows Anticancer Activity against Human Colorectal Cancer Cells: Underlying Molecular Mechanisms
Author(s) -
Abaza Mohamed,
Afzal Mohammad,
AlAttiyah Rajaa,
Guleri Radhika
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.936.10
Subject(s) - apoptosis , colorectal cancer , cyclin dependent kinase 6 , cancer cell , cancer research , cytotoxicity , cancer , growth inhibition , biology , pharmacology , chemistry , cell cycle , biochemistry , cyclin dependent kinase 2 , in vitro , genetics
Natural products have long been used to treat many diseases, including cancer and therefore, these are good candidates for drug development.The present study focuses onthe isolation and identification of potential chemo‐therapeutic/preventive constituents from Tamarix aucheriana using bioactivity guided fractionation. Ferrulic acid methylester (FAME) was identified as an active agent. The potential of FAME to control the growth of human colorectal cancer cells, enhance their sensitivity to conventional chemotherapeutic drugs and the potential biological targets and molecular mechanisms of FAME action were investigated. FAME showed time‐ and dose‐dependent anti‐mitogenic effect on colorectal cancer cells with little cytotoxicity for normal human fibroblasts. FAME inhibited the colony formation, induced morphological changes, induced apoptosis, and inhibited DNA‐binding activity of NFkB, the various proteolytic activities of proteasome and invasion in colorectal cancer cells. FAME up‐regulated p15, p18, p19, p21, p27, p53, and p57, pro‐apoptotic gene expression and down‐regulated Cdk1, Cdk2, Cdk4, and Cdk6, anti‐apoptotic gene expression. FAME showed differential potential to enhance the sensitivity of colorectal cancer cells to conventional chemotherapeutic drugs. These results indicate the chemo‐therapeutic potential of FAME. Acknowledgment: This study was supported by Kuwait University, Research Grant # SL05‐04