Premium
The Mechanistic Enzymology of Thiamin Biosynthesis
Author(s) -
Begley Tadgh
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.93.2
Subject(s) - thiazole , pyrimidine , biosynthesis , biochemistry , chemistry , histidine , enzyme , cysteine , thiamine , pyrimidine metabolism , stereochemistry , glycine , atp synthase , purine , amino acid
My lecture will cover the unusual biosynthesis of the thiazole and pyrimidine heterocycles of thiamin in Saccharomyces cerevisiae . These pathways are fundamentally different from the relatively well‐understood bacterial pathways. The thiamin pyrimidine is formed from PLP and histidine and the thiamin thiazole is formed from NAD, cysteine and glycine. Our studies show that the thiazole sulfur is derived from cysteine 205 of the thiazole synthase (THI4) and the H 2 N‐C=N fragment of the pyrimidine is derived from histidine 66 of the pyrimidine synthase (THI5). Structural and mechanistic studies on these unusual single turnover enzymes will be reported.