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Sustained Delivery of EGCG and TMQ On Fracture Healing Using Male Rats As A Model
Author(s) -
Benghuzzi Hamed,
Tucci Michelle,
Cameron Joseph
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.927.1
Subject(s) - thymoquinone , medicine , alkaline phosphatase , traditional medicine , bone healing , chemistry , surgery , biochemistry , enzyme , antioxidant
The benefits of natural products as an alternative to conventional treatment of various diseases have begun to emerge. Nigella sativa and green tea, natural herbs have been used for many acute as well as chronic conditions. The objectives of this study were (1) to successfully deliver the active components of black seed, thymoquinone (TMQ), and green tea, epigallocatechin‐3‐gallate, or EGCG at sustained levels using a tricalcium phosphate delivery system (TCPL); (2) to evaluate the effects TMQ and EGCG on bone healing (induced femoral defect) in adult male rats. Thirty animals were randomly divided into five equal groups. Groups I and II animals served as control, and sham, while groups III and IV served as TMQ and EGCG experimental groups (0.2 g of TQM or EGCG loaded TCPL). Group V animals served as TMQ (0.2 gm)+EGCG (0.2gm) treated group. Blood, x‐rays and body weights were collected and recorded weekly. Metabolic biomarkers were also evaluated weekly. At the end of 30 days, animals were sacrificed and vital as well reproductive organs were collected and analyzed morphometrically. The results revealed the following: (i) gross anatomical observation indicated an increase in the healing pattern of animals in all experimental groups compared to the sham operated group, (ii) the ease of osteogenesis was in the following order: TMQ+EGCG>TMQ>EGCG>shams, and (iii) no significant differences where detected in the levels of cholesterol, proteins, maliondialdehyde and alkaline phosphatase in all groups. In conclusion, it appears that sustained delivery of TMQ+EGCG can synergistically enhance bone healing compared to TMQ and EGCG treated animals.