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Effects of Flaxseed‐supplementation on DNA Methyltransferase and Obesity‐associated Gene Expression in Adipose Tissue from Obese Mice
Author(s) -
Woodard Alycia,
Rhee Yeong,
Reindl Katie,
Hall Clifford
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.924.8
Subject(s) - adipose tissue , dnmt1 , leptin , gene expression , medicine , endocrinology , dna methyltransferase , obesity , dna methylation , methyltransferase , dnmt3b , chemistry , biology , gene , methylation , biochemistry
Diet‐induced obesity (DIO) has been shown to increase DNA methyltransferase (DNMT) expression and DNMT binding at obesity‐associated genes. Natural compounds, such as flaxseed, have the potential to reverse obesity‐associated gene expression via the regulation of DNA methylation. The objective of this study was to determine the effect of the bioactive compounds of flaxseed on DNMT and obesity‐associated gene expression. Sixty C57BL/6J male mice were randomly assigned into one of the following diet groups and fed for eight weeks: 1) 45% Kcal fat; 2) 45% Kcal fat, 10% whole flaxseed; 3) 45% Kcal fat, 6% defatted flaxseed; 4) 45% Kcal fat, 4% flaxseed oil; and 5) 16% Kcal fat. DNMT1, DNMT3a, DNMT3b, leptin, and peroxisome proliferator‐activated receptor (PPAR)‐α expression levels in mouse adipose tissues were determined by real‐time PCR. DNMT1, DNMT3a, or leptin expression was significantly decreased in whole flaxseed, defatted flaxseed, flaxseed oil, or low fat diet groups compared to the high fat diet group. DNMT1 or DNMT3a expression was positively correlated with PPAR‐α and leptin expression in the defatted flaxseed, flaxseed oil, or low fat diet groups. The results indicate that bioactive compounds of flaxseed affect selected gene expression; however, further research is needed to identify the specific mechanisms regulating leptin or PPAR‐α expression during DIO development.