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Resistant Starch and Lactobacillus Feeding Improve Metabolic Functions in Diet‐Induced Obese Mice
Author(s) -
Barouei Javad,
Mishchuk Darya,
Kieffer Dorothy,
Martinic Alice,
Martin Roy,
Slupsky Carolyn,
Marco Maria
Publication year - 2015
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.29.1_supplement.924.29
Subject(s) - resistant starch , endocrinology , medicine , cecum , gut flora , biology , carbohydrate metabolism , occludin , lipid metabolism , starch , immunology , biochemistry , tight junction
Intestinal microbes have important roles in the regulation of energy homeostasis, lipid and glucose metabolism, and immune system responses. Dysbiotic gut microbiota is associated with obesity, type 2 diabetes mellitus, and metabolic syndrome. Therefore, gut microbiota modulations by fermentable carbohydrates and/or probiotics might protect against these chronic diseases. In this study, male C57BL/6J mice received a high‐fat (HF) diet (45.9% energy from fat) for 9 weeks. Mice were then provided with either a HF diet modified to contain 20% (w/w) type 2 high‐amylose maize resistant starch (HF‐HAMRS) or remained on the control HF diet for another 6 weeks. During that time, half of the mice fed either HF‐HAMRS or HF received supplements of 10^9 cells of Lactobacillus plantarum WCFS1 (Lp) every other day (n=10/grp). HAMRS‐fed mice exhibited enhanced indicators of intestinal fermentation (cecum tissue and content weights and cecal proglucagon gene expression), increased serum adiponectin levels, and sensitized cecal and ileal responses to microbiota changes as indicated by increased expression of TLR genes and ileal antimicrobial Reg 3 g gene. Lp‐fed mice displayed significantly increased hepatic Cpt1a gene expression relative to HF controls, indicating increased fatty acid β‐oxidation. Combining HAMRS and Lp significantly enhanced gene expression of cecal tight junction protein occludin relative to HF control. These outcomes provide a rationale for developing nutritional strategies that prevent or treat obesity and its associated metabolic disorders. Funding: American Diabetes Association.